Using the dCAPS Method to Detect the Resistance of Wild Oat (Avena ludoviciana Durieu.) to Clodinafop-propargyl in Comparison with Current Methods

Document Type : Research Paper

Authors

Abstract

In order to evaluate the resistance of 17 biotypes of wild oat to clodinafop-propargyl herbicides, four methods including: whole plant screening, whole plant bioassay, seedling bioassay and molecular method were used. There was similarity between the results of whole plant screening, whole plant bioassay, seedling bioassay, but resistance ratio (R/S) of seedling bioassay was lower than whole plant bioassay and it seems that seedling bioassay is a reliable method for identifying populations of grass species resistant to ACCase inhibiting herbicides. According to the molecular methods, an isoleucine-1781-leucine [Leu] mutation in plastidic ACCase enzyme of 8 biotypes (44% of biotypes) has been identified as the known mutation endowing clodinafop-propargyl resistance and isoleucine-2041-asparagine mutation has not been identified as a mutation endowing resistance in none of biotype. The resistance mechanism of the rest of biotypes that were resistance according to the non-molecular method, but did not identify resistant by molecular method, can be explained by mutation in another location or metabolism-based mechanism.

Keywords

References

Adkins, S., Wills, W. D., Boersma, M., Walker, S. R., Robinson, G., McLeod, R. J. and Einam, J.P. 1997. Weed resistance to chlorsulfuron and atrazine from the north-east grain region of Australia. Weed Res. 37: 343-349.
Beckie, H. J., Heap, I. M., Smeda, R. J. and Hall, L. M. 2000. Screening for herbicide resistance in weeds. Weed Technol. 14: 428-445.
Bena Kashani, F., Zand, E. and Mohammadalizadeh, H. 2005. Quick test to evolution resistance of wild oat (Avena luduviciana) to aryloxyphenoxypropionate herbicides using seed bioassay. Proceeding of the 1st Iranian Weed Science Congress. Iranian Research Institute of Plant Protection. Tehran. 492-495
Corbett, C. L. and Tardif, F. J. 2006. Detection of resistance to acetolactate synthase inhibitors in weeds with emphasis on DNA-based techniques: a review. Pest Manage Sci, 62: 584-597.
Cullings, K. W. 1992. Design and testing of a plant-specific PCR primer for ecological and evolutionary studies. Mol Ecol. 1: 233-240.
De Prado, R., Gonzalez-Gutierrez, J., Menendez, J., Gasquez, J., Gronwald, J. W. and Gimene-Espinoza, R. 2000. Resistance to acetyl CoA carboxylase-inhibiting herbicides in Lolium multiflorum. Weed Sci. 48: 311-318.
Deihimfard, R., Zand, E.,  Mahdavi Damghani, A. M. and Soufizadeh, S. 2007. Herbicide risk assessment during the wheat self-sufficient project in Iran. Pest Manage Sci. 63: 1036-1045.
Delye, C. 2005. Weed resistance to acetyl coenzyme A carboxylase inhibitors: an update. Weed Sci. 53: 728-746.
Gherekhlo, J. and Zand, E. 2010. A short review on conducted herbicide-resistance researches in Iran. Key articles of 11th Iranian Crop Science Congress. Shahid Beheshti University. Tehran. 110-125.
Kaundun, S. S. and Windass, J. D. 2006. Derived cleaved amplified polymorphic sequence, a simple method to detect a key point mutation conferring acetyl CoA carboxylase inhibitor herbicide resistance in grass weeds. Weed Res. 46: 34-39.
Konieczny, A. and Ausubel, F. M. 1993. A procedure for mapping Arabidopsis mutations using codominant ecotype-specific PCR-based markers. Plant J. 4: 403–410.
Moss, S. R., Clarke, J. H., Blair, A. M., Culley, T. N., Read, M. A., Ryan, P. J. and Turner, M. 1999. To occurrence of herbicide-resistance grass-weeding in the United Kingdom and a new system for designating resistance in screening assay.Proceeding of Brighton Crop Protection Conference on Weeds. Hampshire, UK: BCPC. 179-184.
Moss, S. R., Perryman, S. A. M. and Tatnell, L. V. 2007. Managing herbicide-resistant black grass (Alopecurus myosuroides): Theory and practice. Weed Technol. 21: 300-309.
Neff, M. M., Neff, J. D., Chory, J. and Pepper, A. E. 1998. dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana genetics. Plant J. 14: 387–392.
 
Preston, C. 2009. Herbicide resistance: Target site mutation. In: C. Neal Stewart Jr. Weedy and Invasive Plant Genomics. Wiley Blackwell. Pp. 127-148.
Ritz, C. and Streibig, J. C. 2005. Bioassay analysis using R. J Stat Softw. 12: 1-21.
Sandral, G. H., Dear, B. S., Pratley, J. E. and Cullis, B. R. 1997. Herbicide dose response rate response curve in subterranean clover determined by a bioassay. Aust J Exp Agr. 37: 67-74.
Streibig, J. C. 1988. Herbicide bioassay. Weed Res. 28: 479-4840.
Tal, A., Kotoula, E. and Rubin, B. 2000. Seed bioassay to detect grass weeds resistance to acetyl coenzyme-A inhibiting herbicides. Crop Protect. 467-472.
Yu, Q., Zheng, M., Owen, M., Sattin, M. and Powles, S. B. 2007. Diversity of acetyl- coenzyme A carboxylase mutations in resistance Lolium population: Using clethodim. Plant Physiol. 145: 547-558.
Zand, E., Baghestani, M. A., Nezamabadi, N. and Shimi, P. 2010. The most important herbicides and weeds of Iran. Iran University Press.
Zand, E. and Beckie, H. 2002. Competitive ability of hybrid and open pollination canola (Brassica napus L.) with wild oat (Avena fatua L.).Canadian J. of Plant Sci. 82: 473-480.
Iranian Journal of Weed Science
Volume 6, Issue 1 - Serial Number 1
September 2010
Pages 33-52

Files

Share

How to cite

Statistics