Initial Investigation on Biological Control of Some Important Weed Species of Wheat Fields Using Rhizosphere Fusariums

Document Type : Research Paper

Authors

1 PhD student of Weed Science

2 . Agronomy and Plant Breeding Department, University of Tehran

3 . Plant Protection Department, University of Tehran,

4 Agricultureal Biotechnology Research Institute of Iran

Abstract

In this research, the biological potential of rhizosphere Fusarium (Fusarium spp.) species to control some important weeds of wheat fields was studied. In the greenhouse, wild barely (Hordeum spontaneum), wild oat (Avena ludoviciana), rye (Secale cereale) wild mustard (Sinapis arvensis), and turnipweed (Rapistrum rugosum) seeds were planted on the soils sampled from wheat fileds of East Azerbayejan, Alborz, Tehran, North Khorasan, Razavi Khorasan, Khouzestan, Fars, Qazvin, Qom, Mazandaran, Kermanshah, Hamedan and Golestan provinces. Fusarium species of plants root were isolated. Effect of Fusarium isolates on seedling radicle length was considered as the criteria for successful control (inhibition of radicle length≥ 70%) in the bioassay experiments. In the first step of bioassay experiments, lettuce (Lactuca sativa) seedling was used as a test plant and the next step was carried out on mentioned weed species. Finally, wheat seedling of Sivand cultivar was used to evaluate the host specifity of selected isolates from pervious stages. Isolates with efficient inhibitory effects on weeds with least inhibitory (seedling radicle reduction≥ 10%) effect on wheat, were determined as successful selective biological control agents and identified with morphological and molecular methods. Results showed that one isolation of F. oxysporum on wild mustard and turnipweed, one isolate of F. brachygibbosum on wild barely and one isolate of F. equiseti on wild barely and wild oat, controlled the species very effectively. Knowledge about microbial agenets for biological control of weed species could be helpful in diversifying current management methods and maintenance of efficacy of available chemical herbicides.

Keywords

References

References:
Ahluwalia, A.D. 2007. Bioherbicides: An eco-friendly approach to weed management. Curr. Sci. 92: 10-11.
Abbas, T., Zahir, Z.A. and Naveed, M. 2017. Bioherbicidal activity of allelopathic bacteria against weeds associated with wheat and their effects on growth of wheat under axenic conditions. BioControl. 62: 719-730.
Aldrich, R.J. 1984. Weed-crop ecology: Principles in weed management. Breton Publishers. 456Pp.
Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D.J. 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402.
Aroca, R., Ruiz-Lozano, J.M., Zamarreño, Á.M., Paz, J.A., García-Mina, J.M., Pozo, M.J. and López-Ráez, J.A. 2013. Arbuscular mycorrhizal symbiosis influences strigolactone production under salinity and alleviates salt stress in lettuce plants. J. Plant Physiol. 170: 47-55.
Bacon C.W. and Hinton, D.M. 1996. Symptomless endophytic colonization of maize by Fusarium moniliforme. Can. J. Bot. 74: 1195-1202.
Boari, A. and Vurro, M., 2004. Evaluation of Fusarium spp. and other fungi as biological control agents of broomrape (Orobanche ramosa). Biol. control. 30: 212-219.
Caesar, A.J. 1999. Insect/pathogen interactions are the foundation of weed biocontrol. In: Program Abstracts, X Int. Symp. Biol. Control Weeds. USDAARS and Montana State University, Bozeman, MT. 53 Pp.
Cibichakravarthy, B., Preetha, R., Sundaram, S.P., Kumar, K. and Balachandar, D. 2012. Diazotrophic diversity in the rhizosphere of two exotic weed plants, Prosopis juliflora and Parthenium hysterophorus. World J. Microbiol. Biotechnol. 28:605-613.
Ciotola, M., Watson, A.K. and Hallett, S.G. 1995. Discovery of an isolate of Fusarium oxysporum with potential to control Striga hermonthica in Africa. Weed Res. 35: 303-309.
Derakhshan, A., Najari Kalantar, N., Gherkhlou, J. and Kamkar, B. 2015. Wild mustard (Sinapis arvensis) and turnipweed (Rapistrum rugosum) resistance to herbicide tribenuron-methyl in Agh Ghala. J. Plant Protec. 29: 199-205. (In Persian)
Fröhlich, J., Morin, L., Gianotti, A. and Webster, R. 1999. Exploring the host range of Fusarium tumidum, a candidate bioherbicide for gorse and broom in New Zealand. In Program Abstracts, X X Int. Symp. Biol. Control Weeds. 121 Pp.
Geiser, D.M., del Mar Jiménez-Gasco, M., Kang, S., Makalowska, I., Veeraraghavan, N., Ward, T.J. and O'donnell, K. 2004. FUSARIUM-ID v.1.0: A DNA sequence database for identifying Fusarium. EUR. J. Plant Pathol. 110: 473-479.
Hirsch P.R. and Mauchline T.H. 2012. Mutualism: plant-microorganism interactions. 43–55, in Ogilvie, L.A. and Hirsch P.R. eds. Microbial ecological theory. Norfolk, United Kingdom: Caister Academic Press.
Hoseini, A., Rashed Mohasel, M.H., Kazerouni, E. and Ghalibaf, K. 2015. Evaluation Hordeum spontaneum populations tolerance to herbicide clodinafop-propargyl. J. Plant Protec. 28: 467-473.  (In Persian).
Isack, Y., Benichis, M., Gillet, D. and Gamliel, A., 2014. A selective agar medium for isolation, enumeration and morphological identification of Fusarium proliferatum. Phytoparasitica, 42: 541-547.
Jensen, A.B., Aronstein, K., Flores, J.M., Vojvodic, S., Palacio, M.A. and Spivak, M., 2013. Standard methods for fungal brood disease research. J. Apic. Res. 52: 1-20.
Kremer, R.J. 2013. Interactions between the plants and microorganisms. Allelopathy J. 31: 51-70.
Kremer, R.J. and Kennedy, A.C. 1996. Rhizobacteria as biocontrol agents of weeds. Weed Technol. 10: 601-609.
Kroschel, J., Mueller-Stoever, D., Elzein, A. and Sauerborn, J. 2000. The development of mycoherbicides for the management of parasitic weeds of the genus Striga and Orobanche—A review and recent results. In Proc. X Int. Symp. Biol. Control Weeds.139 Pp. Bozeman, MT: Montana State University.
Ladygina, N. and Hedlund, K. 2010. Plant species influence microbial diversity and carbon allocation in the rhizosphere. Soil Biol. Biochem. 42: 162-168.
Lakshmi, V., Kumari, S., Singh, A. and Prabha, C. 2015. Isolation and characterization of deleterious Pseudomonas aeruginosa KC1 from rhizospheric soils and its interaction with weed seedlings. J. King Saud Univ. Sci. 27: 113-119.
Leslie, J.F., Summerell, B.A. and Bullock, S., 2006. The Fusarium. Laboratory manual, p.388.
Leslie, J.F., Pearson, C.A.S., Nelson, P.E. and Toussoun, T.A. 1990. Fusarium spp. from corn, sorghum, and soybean fields in the central and eastern United States. Pol. J. Ecol. 80: 343-350.
Mirhosseini, H.A., Babaeizad, V. and Hashemi, L., 2014. First report of Fusarium brachygibbosum causing leaf spot on oleander in Iran. J. Plant Pathol. 96:431-435.
Montazeri, M., Zand, E. and Bagheatani, M. A. 2003. Weeds and their control in wheat fields of Iran. Weed Research Department of Research Institute of Pest and Diseases. 85 Pp.
Mortensen, K., 1998. Biological control of weeds using microorganisms. Plant–microbe interactions and biological control. New York: Marcel Dekker. 223-248.
Mrema, E., Shimelis, H., Laing, M. and Bucheyeki, T. 2017. Screening of sorghum genotypes for resistance to Striga hermonthica and S. asiatica and compatibility with Fusarium oxysporum f. sp. strigae. Acta Agr scand B-S P. 67, 395-404.
Nachtigal, G.D.F. and Pitelli, R.A. 1999. Fusarium sp. as a potential biocontrol agent for Egeria densa and Egeria najas. In Program Abstracts, X Int. Symp. n Biol. Control Weeds. 68 Pp.
National Center for Biotechnology Information (NCBI). 1998. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/. Accessed October 25, 2017.
Park, J.M., Radhakrishnan, R., Kang, S.M. and Lee, I.J. 2015. IAA producing Enterobacter sp. I-3 as a potent bio-herbicide candidate for weed control: A special reference with lettuce growth inhibition. Indian J. Microbiol. 55: 207-212.
Pearson, K.A., Taylor, A.F.S., Fuchs, R.M.E. and Woodward, S. 2016. Characterisation and pathogenicity of Fusarium taxa isolated from ragwort (Jacobaea vulgaris) roots. Fungal ecol. 20: 186-192.
Payedar, S., Zand, E., Baghestani, M.A. and Jamali, M.R. 2009. Investigation efficacy of native and foreign Fenoxaprop –p –ethyl for controlling wild Oat (Avena ludoviciana). Iranian J. Weed Res. 1: 45-55. (In Persian with English abstract).
Rahjoo, V., Zad, J., Javan-Nikkhah, M., Gohari, A.M., Okhovvat, S.M., Bihamta, M.R., Razzaghian, J. and Klemsdal, S.S., 2008. Morphological and molecular identification of Fusarium isolated from maize ears in Iran. J. Plant Pathol. 90: 463-468.
Rodriguez, R.J., White Jr, J.F., Arnold, A.E. and Redman, R.S., 2009. Fungal endophytes: Diversity and functional roles. New phytol. 182: 314-330.
Skipper, H.D., Ogg, A.G. and Kennedy, A.C. 1996. Root biology of grasses and ecology of rhizobacteria for biological control. Weed Technol. 10: 610-620.
Stubbs, T.L. and Kennedy, A.C. 2012. Microbial weed control and microbial herbicides. In Herbicides-environmental impact studies and management approaches.InTech.https://www.intechopen.com/ books/herbicides-environmental-impact-studies-and-management-approaches/microbial-weed-control-and-microbial-herbicides. Accessed March 12, 2019.
Te Beest, D.O., Yang, X.B. and Cisar, C.R. 1992. The status of biological control of weeds with fungal pathogens. Annu. Rev. Phytopathol. 30: 637-657.
Tranel, P.J., Gealy, D.R. and Kennedy, A.C. 1993. Inhibition of downy brome (Bromus tectorum) root growth by a phytotoxin from Pseudomonas fluorescens strain D7. Weed Technol. 7: 134-139.
Van Lenteren, J.C., Bolckmans, K., Köhl, J., Ravensberg, W.J. and Urbaneja, A. 2018. Biological control using invertebrates and microorganisms: plenty of new opportunities. BioControl. 63: 39-59.
Wardle, D.A., Yeates, G.W., Williamson, W. and Bonner, K.I. 2003. The response of a three trophic level soil food web to the identity and diversity of plant species and functional groups. Oikos. 102: 45-56.
Westover, K.M., Kennedy, A.C. and Kelley, S.E. 1997. Patterns of rhizosphere microbial community structure associated with co-occurring plant species. J. Ecol.85: 863-873.
Westwood, J.H., Charudattan, R., Duke, S.O., Fennimore, S.A., Marrone, P., Slaughter, D.C., Swanton, C. and Zollinger, R. 2018. Weed management in 2050: Perspectives on the future of Weed Science. Weed Sci. 66: 275-285.
Zand, E., Baghestani, M.A., Nezam Abadi, N., Minbashi Moeini, M. and Hadizadeh, M.H. 2009. A review on the last list of herbicides and the most important weeds of Iran. Iranian J. Weed Res. 2: 83-100.(In Persian with English abstract).
Zhong, S. and Steffenson, B.J. 2001. Virulence and molecular diversity in Cochliobolus sativus. Phytopathology. 91: 469-476.
Iranian Journal of Weed Science
Volume 16, Issue 1
April 2020
Pages 27-36

Files

Share

How to cite

Statistics