Identifying short-spike canarygrass (Phalaris brachystachys) accessions resistant to ACCase-inhibiting herbicides using seed bioassay technique

Document Type : Research Paper

Authors

1 Department of Agronomy, Gorgan University of Agricultural Sciences and Natural Resources, Iran

2 Department of Agricultural Chemistry and Soil Science, University of Córdoba, Spain

3 Center for Scientific and Technological Research of Extremadura (CICYTEX), Badajoz, Spain

Abstract

In order to investigate the resistance of short spike canarygrass (Phalaris brachystachys) biotypes to acetyl coenzyme A carboxylase inhibiting herbicides in wheat fields of Golestan province, 43 suspected resistance were collected from different areas in 2018. The susceptible biotype was also collected from regions with no chemical control record. The study was carried out in three steps including determination of discriminating concentration for susceptible biotype, screening P. brachystachys biotypes with the discriminating concentrations, and concentration-response experiment for suspected resistant biotypes at Gorgan University of Agricultural Sciences and Natural Resources in 2018. According to the seed bioassay test, the discriminating concentration of sensitive biotype for clodinafop propargyl, diclofop methyl, haloxyfop-r-methyl, cycloxydim and pinoxaden were 0.02, 1.36, 0.03, 0.06 and 0.08 mg a.i.L-1, respectively. In the screening test using the discriminating concentration, 36 (84%), 36 (84%), 19 (44%), 23 (53%) and 21 (48%) out of 43 biotypes were identified as suspected resistant to clodinafop propargyl, diclofop methyl, haloxyfop-R-methyl, cycloxydim and pinoxaden, respectively. In general, the results showed that the studied biotypes had different patterns in terms of resistance to different groups of ACCase-inhibiting herbicides. The results of the concentration-response test also showed that among 43 suspected resistant, 19 biotypes were resistant to all five herbicides.

Keywords

References

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Iranian Journal of Weed Science
Volume 16, Issue 2
October 2020
Pages 155-168

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