Evaluation of the Efficacy of some Premixed Herbicides for Controlling Wild Mustard (Sinapis arvensis) Using Chlorophyll a Fluorescence in the Presence and Absence of Salicylic Acid

Authors

1 University of Ardebil

2 Plant Protection Research Department, Hamedan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education, and Extension Organization (AREEO), Hamedan, Iran

10.22034/ijws.2025.135817

Abstract

Chlorophyll a fluorescence is a reliable physiological indicator for determining changes induced in the photosynthetic system and can be altered by various factors that are indirectly involved in photosynthesis. Two greenhouse experiments were conducted to study the effect of salicylic acid (SA) in combination with premixed herbicides including iodosulfuron-methyl + mesosulfuron-methyl sodium + diflufenican (Othello) and pinoxaden + flurasolam (Axial One) on chlorophyll a fluorescence parameters of wild mustard (Sinapis arvensis). The results showed that application of both herbicides decreased the maximum fluorescence (Fm) and the maximum photochemical efficiency of photosystem II (Fv/Fm), while increasing the minimum fluorescence (Fo). Application of Othello at a rate of 528 g a.i. ha⁻¹ resulted in a 30.78% reduction in the Fv/Fm ratio, whereas application of Axial One at 300 g a.i. ha⁻¹ caused a 72.30% reduction, compared to the untreated control (without herbicide and salicylic acid), seven days after herbicide application. Correlation analysis revealed significant relationships between chlorophyll a fluorescence parameters (Fm and Fv/Fm) and weed dry weight under different herbicide treatments. In contrast, minimum fluorescence (F₀) showed a negative correlation with dry weight, with the strongest negative relationship observed under the Axial One treatment. Overall, all herbicide treatments significantly reduced the dry weight of wild mustard, while the application of salicylic acid had no detectable effect on herbicide efficacy. 

Keywords


Volume 21, Issue 1
June 2025
Pages 184-201
  • Receive Date: 15 July 2026
  • Accept Date: 15 July 2026
  • First Publish Date: 15 July 2026
  • Publish Date: 22 June 2025