Using CAPS and dCAPS Methods to Detect some Mutations that Cause Resistance to Acetyl Coenzyme A Carboxylase Inhibiting Herbicides in Wild Oat (Avena ludoviciana)

Document Type : Research Paper

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Abstract

Consecutive usages of acetyl Coenzyme A carboxylase (ACCase) inhibiting herbicides to control troublesome grass weeds of wheat in Iran, has resulted in resistance of 5 grass weeds to ACCase inhibitors. Recently some resistant Avena spp. biotypes have been detected in wheat fields of Iran which have developed resistance to clodinafop propargyl, setoxydim and pinoxaden herbicides with resistance factor between 1.31 to more than 50.63. This experiment was conducted to use CAPS and dCAPS method to detect mutation probably occurred in 1781, 2041, 2078 and 2088 positions of encoding gene of ACCase enzyme and resulted in resistance of resistant wild oat biotypes to ACCase inhibiting herbicides. Results showed that the cause of resistance in 50% of the biotypes (including M4, S2, S3, S4 and ES1) was mutation at the position 1781, 10% (including M2 biotype) at the position 2041 and 10% (including M4) at the position 1781 and 2078. Generally 70% of biotypes showed mutation at least in1781, 2041 and 2078 positions. The cystein-2088-argenin substitutions were not identified as a mutation endowing resistance in any of the biotypes. In other biotypes (M1, M3, S1 and F3) mutation may have occurred in other positions of gene encoding ACCase, or the resistance in these biotypes is a non-target based mechanism.

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References

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Iranian Journal of Weed Science
Volume 9, Issue 1 - Serial Number 1
September 2013
Pages 79-91

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