مطالعه تنوع ژنتیکی درون و بین توده‌های تیغ گرگی Carthamus lanatus در مناطق شمال شرق ایران

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد اصلاح نباتات دانشگاه کشاورزی و منابع طبیعی گرگان

2 استادیار دانشگاه کشاورزی و منابع طبیعی گرگان

3 - استادیار دانشگاه کشاورزی و منابع طبیعی گرگان

چکیده

اطلاع از میزان تنوع ژنتیکی جوامع علف­های‌هرز به روش­های کنترل بیولوژیک آن­ها کمک زیادی می‌کند، مطالعه حاضر، جهت بررسی تنوع ژنتیکی درون و بین توده­های تیغ گرگی (Carthamus lanatus)جمع­آوری شده از هشت رویشگاه مختلف استان گلستان با استفاده از 24 نشانگر  ISSRانجام شد. با استفاده از 9 نشانگری که حدود 04/26 درصد چندشکلی نشان دادند، میانگین ضریب تشابه بین افراد هر توده بر اساس روش جاکارد و پارامترهای توصیفی نظیر تنوع ژنی نئی، شاخص اطلاعات شانون، درصد جایگاه­های چند شکل، تعداد آلل مشاهده شده و تعداد آلل مؤثر محاسبه گردید. بالاترین ضریب تشابه ژنتیکی افراد در توده­های گمیشان و اطراف آن و پایین­ترین آن در توده بندرترکمن وجود داشت. تجزیه و تحلیل واریانس مولکولی نشان داد که میزان تنوع ژنتیکی درون توده­های مورد بررسی (63 درصد) بیش از تنوع ژنتیکی بین توده­ای (23 درصد) بود. بیشترین شاخص تنوع ژنی نئی، اطلاعات شانون، درصد جایگاه­های چند شکل، تعداد آلل مشاهده شده و تعداد آلل مؤثر در توده بندر ترکمن و کمترین آنها در توده­های جزیره آشورآده و اینچه­برون محاسبه شد. بنابراین توده بندرترکمن دارای بالاترین و توده­های جزیره آشورآده و اینچه­برون دارای کمترین مقدار تنوع ژنتیکی درون توده­ای بودند.

کلیدواژه‌ها


عنوان مقاله [English]

A Study of Genetic Variation within and between Ecotypes of Distaff Thistle (Carthamus lanatus) from North East Iran

نویسندگان [English]

  • samane nikdel 1
  • mohammad Hadi Pahlevani 2
  • Khalil Zenalinezhad 3
  • Ahmad Yamchi 2
چکیده [English]

The information of the genetic diversity of weed community helps researchers to find more effective biological control methods. In the present study the genetic diversity within and between populations of distaff thistle (Carthamus lanatus) was investigated using 24 ISSR markers. The populations were collected from eight different regions of Golestan province. Using 9 polymorphic (26.4 %) ISSR markers, the mean coefficient of similarity between individuals based on Jaccard, and descriptive parameters such as Neis gene diversity, Shannon's information index, percentage of polymorphic loci, observed number of alleles (na) and effective number of alleles (ne) were calculated for each population. The highest genetic similarity coefficient was observed in the ecotypes collected around Gomishan and the lowest occurred in ecotype Bandartorkman. Analysis of molecular variance showed that the genetic diversity among populations (63 percent) was higher than genetic variation between populations (23 percent). The highest values for Nei’s gene diversity, Shannon's information index, percentage of polymorphic loci, observed number of alleles, effective number of alleles was observed in ecotype Bandartorkman and the lowest values of these parameters occurred for ecotypes AshuradehIsland and Inchehbroon. Therefore, ecotype Bandartorkman had the highest, and ecotypes of AshuradehIsland and Inchehbroon the lowest within population genetic diversity.

کلیدواژه‌ها [English]

  • Analysis of molecular variance
  • biological control
  • coefficient of similarity
  • ISSR
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