نوع مقاله : مقاله پژوهشی
نویسنده
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسنده [English]
The present experiment set out to rapidly detect some mechanisms of resistance to the herbicide tribenuron-methyl in Sinapis arvensis. For this purpose, a chitosan microneedle was utilized to extract DNA, and then, to detect mutations, sequencing of the acetolactate synthase (ALS) gene through PCR product was carried out. During a separate process, the insecticide malathion was also used to determine the metabolic resistance mechanism. The results of the present study indicated that using the microneedle reduced the DNA extraction time to five minutes compared to the conventional approach. The ALS gene sequences in the FR3 (resistant) and S (susceptible) populations did not differ from the sensitive sequences available in GenBank; therefore, no mutations were observed in the gene. In a dose-response experiment with different doses of the herbicide tribenuron-methyl (up to eight times the recommended dose; that is, 20 grams of active ingredient per hectare), applied with pretreatment of malathion (a dose of 1000 grams of active ingredient per hectare) two hours before the herbicide treatments, and without malathion pretreatment as well, the resistant FR3 population had 23 times more resistance than the susceptible (S) population in the presence of tribenuron-methyl and without malathion pretreatment. Pretreatment with malathion significantly
(p≤ 0.01) reduced herbicide resistance (RI= 1.15) in the FR3 population, which indicated metabolism-based resistance to tribenuron-methyl.
کلیدواژهها [English]
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