مطالعه تفاوت ژنتیکی توده‌های مختلف علف‌هرز جو دره Hordeum spontaneum با استفاده از نشانگر SSR

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار گروه زراعت و اصلاح نباتات دانشگاه کردستان

2 عضو هیئت علمی دانشگاه تهران

3 موسسه تحقیقات گیاهپزشکی

4 دانشگاه تهران

5 دانشجوی دکتری اصلاح نباتات دانشگاه ایلام

چکیده

به‌منظور بررسی تفاوت ژنتیکی توده‌های مختلف جودره در ایران، پژوهشی طی سال‌‌های 93-1392 در گروه زراعت و اصلاح نباتات دانشگاه تهران انجام شد. در این پژوهش 40 توده جمع‌آوری‌شده از سراسر کشور با استفاده از 11 نشانگر SSR مورد بررسی قرار گرفتند. نتایج به‌طور کل نشان داد که تعداد باند‌های چند شکل از 2 تا 14 باند برای هر آغازگر متغیر بود. به‌طور متوسط 7 باند و 6/5 باند چند شکل برای همه آغازگرها حاصل شد. از کل واریانس مشاهده شده، در حدود 48 درصد تنوع مشاهده شده مربوط به تنوع بین جمعیت‌ها و 52 درصد مربوط به داخل جمعیت‌ها بود که مقدار تمایز بالای بین جمعیت‌ها را نشان می‌دهد. 40 توده مورد بررسی جودره در 5 گروه مختلف قرارگرفتند. گروه الف) شامل توده‌های استان فارس، گروه ب) شامل توده‌های یزد، چهارمحال و بختیاری، لرستان، ایلام و یک توده همدان، گروه ج) شامل توده‌های قزوین، البرز، خمین، قم و خراسان، گروه د) شامل توده‌های کردستان، آذربایجان غربی، اردبیل و قزوین و گروه هـ) شامل توده‌های همدان، کردستان و کرمانشاه شدند. قرارگیری توده‌های مناطق نزدیک به هم در یک گروه، ممکن است به دلیل نفوذ این توده‌ها به داخل همدیگر، از طریق ماشین آلات کشاورزی و یا بذور گیاهان زراعی باشد. همچنین به ‌احتمال زیاد فرضیه انتقال بذر جودره از استان فارس، حداقل به مناطق دوردست مانند کرج و ورامین مردود است. آگاهی از تنوع ژنتیکی این علف‌هرز، می‌تواند منجر به پیشرفت برنامه‌های مدیریتی به منظور کنترل مؤثر این علف‌هرز مخصوصا در مزارع گندم شود.

کلیدواژه‌ها


عنوان مقاله [English]

Analysis of Genetic Diversity in Wild barley (Horedeum spontaneum) Populations by Using SSR Marker

نویسندگان [English]

  • sirvan babaei 1
  • Hassan Alizadeh 2
  • Mohammad ali baghestani 3
  • Mohammad reza Naghavi 4
  • sohila mohammadi 5
2 University of Tehran
چکیده [English]

In order to investigate genetic variation of Hordeum spontaneum C. Koch populations in Iran, an experiment was conducted at the research section of the Department of Agronomy and Plant Breeding, University of Tehran from 2013 to 2014. In this study, 40 ecotypes of H. spontaneum collected from all parts of Iran were investigated by means of 11 SSR markers. Results revealed that the number of polymorphic bands for each primer varied from 2 to 14, on average, 7 and 5.6 polymorphic bands for all primers. Total variances observed were related to diversity among and within the populations which were 48% and 52% respectively. This means a high differentiation among the populations. The biotypes of wild barely (40) were divided into 5 groups including: A: Fars populations, B: populations of Yazd, Chaharmahal va bakhtiari, Lorestan, Ilam and Hamedan populations, C: Qazvin, Alborz, Khomein, Qom and Khorasan populations, D: Kurdistan, West Azarbaijan, Lorestan, Ardebil and Qazvin populations. E: Hamedan, Kurdistan and Kermanshah populations. The placement of closed area populations in one group may be due to penetrations into each other, through farm machinery or cultivated crop seeds. The hypothesis of seed transport from Fars province to far areas such as Karaj and Varamin is rejected. Knowledge of the genetic diversity of this weed, can lead to development of management plans in order to effectively control this weed especially in wheat fields.

کلیدواژه‌ها [English]

  • Genetic diversity
  • SSR marker
  • weed dispersal
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