استفاده از روش‌های CAPS و dCAPS در تشخیص برخی جهش‌های منجر به مقاومت یولاف وحشی (Avena ludoviciana) به علف‌کش‌های بازدارنده استیل کوآنزیم آ کربوکسیلاز

نوع مقاله : مقاله پژوهشی

نویسندگان

1 موسسه تحقیقات گیاهپزشکی

2 دانشگاه صنعتی اصفهانی

3 دانشگاه گرگان

چکیده

کاربرد مداوم علف‌کش‌های بازدارنده‌استیل کوآنزیم‌آ کربوکسیلاز برای کنترل علف‌های هرز باریک‌برگ‌های مشکل‌ساز در مزارع گندم کشور منجر به بروز مقاومت در 5 گونه از علف‌های هرز باریک‌برگ شده است. اخیرا بیوتیپ‌هایی از علف هرز یولاف وحشی مقاوم به علف‌کش کلودینافوپ پروپارژیل، ستوکسیدیم و پینوکسادن مورد شناسایی قرار گرفته که دارای شاخص مقاومتی بین 31/1 تا بیش از 63/50 در برابر بازدازنده های ACCase بودند. در این آزمایش اساس ملکولی مقاومت در بیوتیپ‌های مقاوم مورد بررسی قرار گرفت. بدین منظور از روش‌های CAPS و dCAPS برای شناسایی جهش‌های احتمالی که در نقاط 1781، 2041، 2078 و 2088 ژن کدکننده آنزیم ACCase رخ داده و باعث مقاومت یولاف وحشی به علف‌کش‌های بازدارنده استیل کو آنزیم آ کربوکسیلاز شده‌اند، استفاده شد. نتایج نشان داد که علت مقاومت در 50 درصد بیوتیپ ‌(شامل بیوتیپ های M4، S2، S3، S4 و ES1) جهش در موقعیت 1781، در 10 درصد بیوتیپ ها (شامل بیوتیپ M2 ) جهش در موقعیت 2041 و در 10 درصد بیوتیپ ها (شامل بیوتیپ M4‌) جهش در دو جایگاه 1781 و 2078 کربوکسیل ترانسفراز آنزیم استیل کوآنزیم آ کربوکسیلاز بود. در مجموع 70 درصد بیوتیپ های مقاوم حداقل در یکی از نقاط 1781، 2041، 2078 جهش داشتند و در هیچ یک از بیوتیپ‌های مورد مطالعه جهش در موقعیت 2088 مشاهده نشد. در سایر بیوتیپ‌های مقاوم، احتمالا جهش در نقطه دیگری از ژن کدکننده آنزیم ACCase رخ داده و یا مکانیزم مقاومت آنها مبتنی بر غیر هدف می‌باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Using CAPS and dCAPS Methods to Detect some Mutations that Cause Resistance to Acetyl Coenzyme A Carboxylase Inhibiting Herbicides in Wild Oat (Avena ludoviciana)

نویسندگان [English]

  • eskandar zand 1
  • Arash Razmi 1
  • fatemeh benakashani 2
  • noushin nezamabadi 1
  • javid gherekhloo 3
  • hamid reza sasanfar 1
1
2
3
چکیده [English]

Consecutive usages of acetyl Coenzyme A carboxylase (ACCase) inhibiting herbicides to control troublesome grass weeds of wheat in Iran, has resulted in resistance of 5 grass weeds to ACCase inhibitors. Recently some resistant Avena spp. biotypes have been detected in wheat fields of Iran which have developed resistance to clodinafop propargyl, setoxydim and pinoxaden herbicides with resistance factor between 1.31 to more than 50.63. This experiment was conducted to use CAPS and dCAPS method to detect mutation probably occurred in 1781, 2041, 2078 and 2088 positions of encoding gene of ACCase enzyme and resulted in resistance of resistant wild oat biotypes to ACCase inhibiting herbicides. Results showed that the cause of resistance in 50% of the biotypes (including M4, S2, S3, S4 and ES1) was mutation at the position 1781, 10% (including M2 biotype) at the position 2041 and 10% (including M4) at the position 1781 and 2078. Generally 70% of biotypes showed mutation at least in1781, 2041 and 2078 positions. The cystein-2088-argenin substitutions were not identified as a mutation endowing resistance in any of the biotypes. In other biotypes (M1, M3, S1 and F3) mutation may have occurred in other positions of gene encoding ACCase, or the resistance in these biotypes is a non-target based mechanism.

کلیدواژه‌ها [English]

  • Clodinafop-propargyl
  • pinoxaden
  • Restriction enzyme
  • setoxydim

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