اثر ترکیب برومایسید آ-ام با سولفات آمونیوم، سیتوگیت و سالیسیلیک اسید بر فلورسانس کلروفیل a تاج‌خروس ریشه‌قرمز (.Amaranthus retroflexus L)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 فارغ‌التحصیل دکتری علوم علف‌های‌هرز، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی ، دانشگاه تهران

2 استاد گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی ، دانشگاه تهران

3 دانشیار علوم علف‌های‌هرز، گروه اکوفیزیولوژی گیاهی، دانشکده کشاورزی، دانشگاه تبریز

چکیده

به منظور ارزیابی اثرات کاربرد برومایسید آ-ام به تنهایی و در ترکیب با سالیسیلیک اسید، سیتوگیت و سولفات آمونیوم بر فلورسانس کلروفیل a تاج‌خروس ریشه قرمز، آزمایش گلخانه‌ای در سال 1396، در قالب طرح بلوک­های کامل تصادفی با سه تکرار، در گلخانه دانشکده کشاورزی دانشگاه تبریز، اجرا شد. علف­کش برومایسید آ-ام، 16 ساعت پس از کاربرد، سبب افزایش 84 درصدی فلورسانس حداقل (Fo)، 89 درصدی هدرفت انرژی به صورت گرما (F0/Fm)و 59 درصدی میزان تجمع مراکز واکنش بسته شده (∆V/∆to) و کاهش 40 درصدی فلورسانس حداکثر (Fm)، 71 درصدی حداکثر کارایی فتوشیمیایی اولیه فتوسیستم II (Fv/Fm)، 94 درصدی عملکرد کوانتومی انتقال الکترون القائی از QA- به پلاستوکوئینون(φEo)، 97 درصدی انرژی لازم برای بسته شدن مراکز واکنش (Sm) و 89 درصدی فاصله زمانی بین فلورسانس حداقل و حداکثر (Tfm) شد. سالیسیلیک اسید به عنوان تنظیم کننده رشد گیاهی، اثر منفی بر کارایی برومایسید آ-ام نداشت. 64 ساعت بعد از کاربرد علف­کش، سولفات آمونیوم و سیتوگیت با اثر سینرژیست روی برومایسید آ-ام، به ترتیب باعث کاهش 52 و 45 درصدی Fm، 62 و 37 درصدی Fv/Fm، 25 و 11 درصدی  Fv/F0 و افزایش 6 و 5/3 درصدی F0/Fm، 72 و 25 درصدی جریان جذب شده در هر مرکز واکنش (ABS/RC) و 83 و 30 درصدی هدررفت انرژی در هر مرکز واکنش در زمان شروع (DI0/RC) شدند. نتایج نشان داد که ارزیابی فلورسانس کلروفیل a می­تواند، روشی سریع و غیرتخریبی برای بررسی پاسخ سیستم فتوسنتزی گیاه به کاربرد علف­کش به تنهایی یا در ترکیب با مواد افزودنی باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Chlorophyll a fluorescence of redroot pigweed (Amaranthus retroflexus L.) in response to mixture of Bromicide AM with Ammonium Sulphate, Cytogaite and Salicylic acid

نویسندگان [English]

  • Soheila Porheidar Ghafarbi 1
  • Hamid Rahimian 2
  • Hassan Alizadeh 2
  • Sirus Hassan Nejad 3
1 Department of Agronomy and Plant Breading, Faculty of Agriculture, University of Tehran
2 Department of Agronomy and Plant Breading, Faculty of Agriculture, University of Tehran
3 Department of Plant Eco-Physiology, Faculty of Agriculture, University of Tabriz, Iran
چکیده [English]

To evaluate the effects of bromicide AM (bromoxynil + MCPA)alone or mixed by salicylic acid, cytogaite, and ammonium sulphate on chlorophyll a fluorescence (ChlF) of redroot pigweed (Amaranthus retroflexus L.), a greenhouse experiment was conducted in randomized complete block design with three replications in Agricultural Faculty of University of Tabriz in 2017. Bromicide AM, 16 hours after application, increased minimum fluorescence (F0) 84%, thermal dissipation quantum yield (F0/Fm) 89% and the rate of accumulation of closed reaction centers (∆V/∆to) 59% and decreased maximum fluorescence (Fm) 40%, maximum quantum yield of photochemistry (Fv/Fm) 71%, electron transport yield (φEo) 94%, normalized area (Sm) 97% and time needed to reach Fm (Tfm) 89%. Salicylic acid as a growth regulator had not negative effect on the efficacy of bromicide AM. 64 hours after herbicide application, synergistic effect on bromicide AM ammonium sulphate and cytogaite reduced 52% and 45% Fm, 62% and 37% Fv/Fm, 25% and 11% Fv/ F0, and enhanced 6% and 3.5% F0/Fm, 72% and 25% specific fluxes expressed per reaction centers (ABS/RC), 83% and 30% ratio – flux of dissipated energy per reaction center in t = 0, respectively. Results showed that assessment of chlorophyll a fluorescence is a rapid and non-destructive technique for detecting photosynthetic apparatus response of plant to applied herbicide alone and or mixing with adjuvants.

کلیدواژه‌ها [English]

  • Adjuvant
  • chlorophyll a fluorescence
  • herbicide
  • salicylic acid
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