بررسی تنوع ژنتیکی توده های مختلف نی در ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری دانشگاه تهران

2 استاد دانشگاه تهران

چکیده

نی از علف های هرز چندساله است که وسیع ترین پراکنش را در بین گیاهان گلدار دنیا دارد. 39 جمعیت نی در ایران جمع آوری شد و تنوع  ژنتیکی بین آنها با استفاده از نشانگرهای RAPD  بررسی شد. 16 پرایمر 149 باند تولید کردند که 82% آن یعنی 123 باند، پلی مورفیک بود. ضرایب شباهت ژنتیکی بین جمعیت ها از 44/0تا 84/0 متغیر و متوسط شباهت ژنتیکی 60/0 بود. نتایج آزمایش نشان داد که نشانگرهای RAPD برای بررسی تنوع ژنتیکی نی، مناسب بودند. هچنین شباهت ژنتیکی بین جمعیت ها اهمیت تکثیر رویشی را در این علف هرز نشان می دهد.

عنوان مقاله [English]

Analysis of Genetic Diversity among Populations of Common Reed (Phragmites australis) in Iran

نویسندگان [English]

  • Marjan Dianat 1
  • Ali Akbar Shahnejat Boshehri 2
  • Hassan Alizadeh 2
  • Mohammad Reza Naghavi 2
  • Hamid Rahimian mashadi 2
1 Phd Student, University of Tehran
2 University of Tehran
چکیده [English]

The common reed (Phragmites australis) is a perennial weed with the largest geographical distribution of any flowering plant in the world. In this study, 39 populations of common reed were collected and random amplified polymorphic DNA (RAPD) markers were used to characterize the genetic diversity among these populations. The 16 primers used in this study, amplified 149 scorable RAPD bands among which 123 were polymorphic (82%). Genetic similarity coefficients ranged from 0.44 to 0.84, with an average of 0.60. Results showed that RAPD markers are suitable for the evaluation of genetic diversity of common reed. The results support the clonal nature of P.australis with its growth and spread primarily by vegetative propagation

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