جهش‌ در آنزیم استیل کوآنزیم آ کربوکسیلاز، مکانیسم مقاومت عرضی بیوتیپ‌های یولاف وحشی(Avena ludoviciana Deuri.) به علف‌کش‌های بازدارنده ACCase

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه صنعتی اصفهان

2 موسسه تحقیقات گیاهپزشکی

3 دانشگاه تهران

4 دانشگاه فردوسی مشهد

چکیده

به منظور تشخیص مکانیزم احتمالی مقاومت براساس محل عمل و نوع جهش‌های صورت گرفته در آنزیم ACCase، 13 بیوتیپ یولاف وحشی(Avena ludoviciana Deuri.)  جمع‌آوری شده از استان خوزستان که دارای مقاومت عرضی به علفکش‌های بازدارنده ACCase بودند آزمایشات مولکولی انجام شد. در این آزمایشات جهش‌های مهم  در محل کربوکسیل ترانسفراز آنزیم ACCase (شامل چهار جهش ایزولوسین-2041-آسپاراژین، سیستین-2088-آرژنین، ایزولوسین-1781-لوسین و آسپارتیک اسید-2078-گلایسین) مورد بررسی قرارگرفتند. برای انجام این مطالعه از روش‌های CAPS و dCAPS استفاده شد. هردو روش شامل سه مرحله استخراج  DNAاز بیوتیپ‌‌های مورد آزمایش،PCR با استفاده از پرایمرهای اختصاصی و در نهایت هضم توسط آنزیم برشی اختصاصی بود. نتایج این تحقیق نشان داد که 8/53 درصد بیوتیپ‌‌های مقاوم به بازدارنده‌های ACCase دارای جهش ایزولوسین-1781-لوسین و 6/7 درصد بیوتیپ‌‌ها دارای جهش آسپارتیک اسید-2078-گلایسین‌می‌باشند. در بین بیوتیپ‌های مورد بررسی تنها یک بیوتیپ دارای دو جهش (ایزولوسین-1781-لوسین و آسپارتیک اسید-2078-گلایسین) بود و در هیچکدام از بیوتیپ‌های مورد مطالعه جهش  ایزولوسین-2041-آسپاراژین و سیستین-2088-آرژنین دیده نشد. بنابراین الگوهای مختلف مقاومت عرضی بیوتیپ‌های یولاف وحشی به دلیل وجود جهش‌های مختلف در ناحیه کربوکسیل ترانسفراز آنزیم ACCase می‌باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Mutations in Acetyl-CoA Carboxylase Enzyme, Mechanism of Cross Resistance in Wild Oat (Avena ludoviciana Deuri.) Biotypes to ACCase Inhibitor Herbicides

نویسندگان [English]

  • Fatemeh Benakashani 1
  • Eskandar Zand 2
  • Mohammad Reza Naghavi 3
  • Hamid Reza Sasanfar 4
چکیده [English]

In order to detect the probability of ACCase inhibitor herbicides target-site based resistance of 13 wild oat (Avena ludoviciana Deuri.) biotypes, molecular experiments were performed using CAPS and dCAPS methods. Biotypes were collected from khuzestan province and which showed cross-resitance to ACCase inhibitors. The above-mentioned molecular methods were used to identify the locations of four important mutations (Isoleucine-2041-Asparagine, Cystein-2088-Arginin, Isoleucine-1781-Leucine and Aspartic acid-2078-Glycine) responsible for target site based herbicide resistance in the carboxyltransferase domain of ACCase enzyme. Both approaches involve DNA extraction, PCR using specific primers and restriction digests of PCR products with restriction enzymes. Results of experiments showed that Isoleucine-1781-Leucine and Aspartic acid-2078-Glycine mutations were observed in 53.8% and 7.6% of resistant wild oat biotypes, respectively. Among the investigated biotypes, only one biotype had two mutations (Isoleucine -1781-Leucine and Aspartic acid-2078-Glycine). Thus, different mutations in the carboxyl transferase domain of ACCase enzyme were the reason of various cross resistance patterns among wild oat biotypes.

کلیدواژه‌ها [English]

  • Mutation
  • Target site-based resistance
  • ACCase enzyme
  • CAPS
  • dCAPS

مراجع

Barth, S., Melchinger, A.E. and Luebberestedt, T. 2002. Genetic diversity in Arabidopsis thaliana L. Heynh. Investigated by cleaved amplified polymorphic sequence (CAPS) and intersimple sequence repeat (ISSR) markers. Mol. Ecol. 11:495-505.
Beckie, H.J. and Kirkland, K. J. 2003. Implication of reduced herbicide rates on resistance enrichment in wild oat (Avena fatua). Weed Technol. 17:138-148.
Benakashani, F., Zand, E. and Naghavi, M. R. 2010. Investigation of the cross resistance to ACCase inhibitor herbicides in wild oat (Avena ludoviciana Durieu.) populations from Khuzestan province and chemical control of resistant populations. Iran. J. of Weed Sci. 6 (1):95-112.
Bourgeois, L., Kenkel, N.C. and Morrison, I.N. 1997. Characterization of cross resistance patterns in acetyl-CoA carboxylase inhibitor resistant wild oat (Avena fatua). Weed Sci. 45:750-755.
Bradley, K.W., Wu, J., Hatzios, K.K. and Hagood, E.S. 2001. The mechanism of resistance to aryloxyphenoxypropionate and cyclohexanedion herbicides in a Johnsongrass biotype. Weed Sci. 49: 477-484.
Brown A.C., Moss, S.R., Wilson, Z.A. and Field, L.M. 2002. An isoleucine to leucine substitution in the ACCase of Alopecurus myosuroides (black grass) is associated with resistance to the herbicide sethoxydim. Pestic Biochem Phys. 72:160–168.
Christoffers M.J., Berg, M.L. and Messersmith, C.G. 2002. An isoleucine toleucine mutation in acetyl-CoA carboxylase confers herbicide resistance in wild oat. Genome. 45:1049-1056.
Christoffers, M.J. 1999. Genetic aspects of herbicide resistant weed management. Weed Technol. 13: 647-652.
Cullings, K.W. 1992. Design and testing of a plant-specific PCR primer for ecological and evolutionary studies. Molecular Ecology. 1:233-240.
De´lye C., Zhang, X.Q., Michel, S., Mate´jicek, A. and Powles, S. B. 2005. Molecular bases for sensivity to acetyl-coenzyme A carboxylase inhibitors in black-grass. Plant Physiol. 137:794-806.
De´lye, C. 2005. Weed resistance to acetyl coenzyme A carboxylase inhibitors: an update. Weed Sci. 53:728-746.
De´lye, C. and Michel, S. 2005. 'Universal' primers for PCR-sequencing of grass chloroplastic acethyl-CoA carboxylase domains involved in resistance to herbicides. Weed Res. 45:323-330.
De´lye, C., Calmes, E. and Mate´jicek, A. 2002a. SNP markers for black-grass (Alopecurus myosuroides Huds) genotypes resistance to acetyl-CoA carboxylase inhibiting herbicides. Theor Appl Genet. 104:1114–1120.
De´lye, C., Mate´jicek, A. and Gasquez, J. 2002b. PCR-based detection of resistance to acetyl-CoA carboxylase-inhibiting herbicides in black-grass (Alopecurus myosuroides Huds) and ryegrass (Lolium rigidum Gaud). Pest manag Sci. 58:474-478.
De´lye, C., Wang, T.Y. and Darmency, H. 2002c. An isoleucine-leucine substitution in chloroplastic acetyl-CoA carboxylase from green foxtail (Setaria viridis L. Beauv.) is responsible for resistance to the cyclohexanedione herbicide sethoxydim. Planta. 214:421–427.
De´lye, C., Zhang, X.Q., Chalopin, C., Michel, S. and Powles, S.B. 2003. An isoleucine residue within the carboxyl-transferase domain of multidomain acetyl-coenzyme A carboxylase is a major determinant of sensitivity to aryloxyphenoxypropionate but not to cyclohexanedione inhibitors. Plant Physiol. 132:1716-1723.
Doyle, J.J. and Doyle, J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry. 19:11-15.
Gherekhloo, J., Rashed Mohassel, M. H., Nasiri Mahalati, M., Zand, E., Ghanbari, A., Osuna, M.D., Ruiz-Santaella, J. P., Wagner, J. and De Prado,R. 2008. ACCase mutations confer ACCase resistance in two Phalaris minor populations from Iran. The proceeding of the 5th International Weed Sci. Vancouver, Canada.
Hassan, C., Muller-Warrant, G. and Griffith, S. 2002. Differential sensivity of Italian ryegrass (Lolium Multiflorum) cultivars to fenoxaprop. Weed Sci. 50:567-575.
Kaundun, S.S. and Windass, J.D. 2006. Derived cleaved amplified polymorphic, a simple method to detect a key point mutation conferring acethyl CoA carboxylase inhibitor herbicide resistance in grass weeds. Weed Res. 46:34-39.
Kuk, Y.I., Burgos, N.R. and Talbert, R.E. 2000. Cross - and multiple resistance of dic1ofop- resistant Lolium spp. Weed Sci. 48:412-419.
Liu, W.J., Harrison, D.K., Chalupska, D., Gornicki, P., O’Donnell, C.C., Adkins, S.W., Haselkorn, R. and Williams, R.R. 2007. Single-site mutations in the carboxyltransferase domain of plastid acetyl-CoA carboxylase confer resistance to grass-specific herbicides. Proc. Natl. Acad. Sci. USA. 104:3627-3632.
Neff, M.M., Neff, J.D., Chory, J. and Pepper, A.E.1998. dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana genetics. Plant J. 14:387-392.
Powles, S.B., Peterson, C., Bryan, I.B. and Jutsum, A.R. 1997. Herbicide resistance: Impact and management. Adv. Agron. 58:57-93.
Preston, C. and Mallory-Smith, C.A. 2001. Biochemical mechanisms, inheritance and molecular genetics of herbicide resistance in weeds. Pages 23-60 in S. B. Powles and D. L. Shaner, eds. Herbicide resistance and world grains. Boca Raton, FL: CRC Press.
Seefeldt, S.S., Gealy, D.R., Brewter, B.D. and Fuerst, E.P. 1994. Cross-resistance of several diclofop-resistant wild oat (Avena fatua) biotypes from the Willamette Valley of Oregon. Weed Sci. 42:430-437.
Tal, A. and Rubin, B. 2004. Molecular characterization and inheritance of resistance to ACCase herbicides in Lolium rigidum. Pest Manag. Sci. 60:1013-1018.
Uludag, A., Park, K.W., Cannon, J. and Mallory-Smith, A. 2008. Cross resistance of Acetyle-CoA carboxylase (ACCase) inhibitor-resistant wild oat (Aven fatua) biotypes in the Pacific Northwest. Weed Technol. 22:142-145.
Vila-Aiub, M.M., Neve, P. and Powles, S. B.2009. Fitness costs associated with evolved herbicide resistance alleles in plants. New Phytol. 184:751–767.
White, G.M., Moss, S.R., Karp, A. 2005. Differences in the molecular basis of resistance to the cyclohexanedione herbicide sethoxydim in Lolium multiflorum. Weed Res. 45:440-448.
Yu, Q., Collavo, A., Zheng, M.Q., Owen, M., Sattin, M. and Powles, S B. 2007. Diversity of Acetyl-Coenzyme A Carboxylase Mutation in Resistant Lolium Populations: Evaluation Using Clethodim. Plant Physiol. 145:547-558.
Zagnitko, O., Jelenska, J., Tevzadze, G., Haselkorn, R. and Gornicki, P. 2001. An isoleucine/leucine residue in the carboxyltransferase domain of acetyl- CoA carboxylase is critical for interaction with aryloxyphenoxypropionate and cyclohexanedione inhibitors. Proc National Academy Science, USA 98:6617-6622.
Zand, E., Razmi, A., Benakashani, F., Nezamabadi, N., Gharkhloo, J. and Sasanfar, H.M. 2013. Using CAPS and dCAPS methods to detect some mutations that cause resistance to acetyl coenzyme A carboxylase inhibiting herbicides in wild oat (Avena ludoviciana). Iran. J. of Weed Sci. 9:79-91.
Zhang, X.Q. and Powles, S.B. 2006a. Six amino acid substitutions in the carboxyltransferase domain of the plastidic acetyl-CoA carboxylase gene are linked with resistance to herbicides in a Lolium rigidum population. New Phytol. 172:636-645.
Zhang, X.Q. and Powles, S.B. 2006b. The molecular bases for resistance to acetyl coenzyme A carboxylase (ACCase) inhibiting herbicides in two target based resistant biotypes of annual ryegrass (Lolium rigidum). Planta. 223:550-557.

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